Hypothetical example $($will repeat for remaining problems $$ You have received a new molecule where someone tried to develop a liquid formulation but was not able to provide adequate stability. Their best formulation contained $20$ mg$/$mL protein $(2\%)$ in $20$ mM histidine $(0\mathrm{.}3\%),85$ mM NaCl $(0\mathrm{.}5\%),3\%$ sorbitol, pH $6\mathrm{.}0?$

What is the glass transition temperature of this formulation $($assume Tg$\text{'}$ of protein is $-10$ C$,$ sodium chloride is $-120$ C$).$ Only enter the number $($to one decimal place$)$ in degrees Celsius $?$

firstly we have to convert everything to mg$/$ml and then we will get the value of w$1$ and w$2$ and then put the glass transition formula to calculate the appropriate answer.