Question: I need help eith this restriction enzymes homework please 5 G GATCC 3 3' CCTAGG 5 Bam HI 5 GAATTC 3 3' CTTAAG 5 EcoRI

I need help eith this restriction enzymes homework please
I need help eith this restriction enzymes homework please 5 G GATCC

5 G GATCC 3 3' CCTAGG 5 Bam HI 5 GAATTC 3 3' CTTAAG 5 EcoRI Haelll 5 GGCC 3 3 CCGG 5 1 5' GCGC 3 3' CGCG 5 Hhal 5' CTCGAG 3 3 GAGCTC 5 Xhol i) ii) iii) Which restriction enzymes do not leave any single stranded DNA after cutting (blunt end)? Which restriction enzymes leave single stranded DNA after cutting sticky end)? Why do you think they are called sticky ends? If I cut a plasmid with EcoRI and then my proposed insert with Xhol will they ligate (join) together? If I cut a plasmid with EcoRI and then my proposed insert with EcoRI will they ligate (join) together? iv) V)

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer
Step: 1 Unlock blur-text-image
Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock
Step: 3 Unlock

Students Have Also Explored These Related Chemical Engineering Questions!