Question: I need help eith this restriction enzymes homework please 5 G GATCC 3 3' CCTAGG 5 Bam HI 5 GAATTC 3 3' CTTAAG 5 EcoRI
5 G GATCC 3 3' CCTAGG 5 Bam HI 5 GAATTC 3 3' CTTAAG 5 EcoRI Haelll 5 GGCC 3 3 CCGG 5 1 5' GCGC 3 3' CGCG 5 Hhal 5' CTCGAG 3 3 GAGCTC 5 Xhol i) ii) iii) Which restriction enzymes do not leave any single stranded DNA after cutting (blunt end)? Which restriction enzymes leave single stranded DNA after cutting sticky end)? Why do you think they are called sticky ends? If I cut a plasmid with EcoRI and then my proposed insert with Xhol will they ligate (join) together? If I cut a plasmid with EcoRI and then my proposed insert with EcoRI will they ligate (join) together? iv) V)
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