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TABLE 5.2: Absorbance Values for Various Concentrations of Proteins Test tube Volume of Biuret Reagent Absorbance [Abs-log(T) Concentration Volume and Concentration of Protein Sample 3 mls of 2000 mg/ml BSA) 1 3 mls 2 3 mLs 3 3 mLs 0.92 11000 Halme 0.56 500 g/ml 0.4 25049/my 0.21125, my -0.02 1.24 4 3 mLs of 1000 (ug/mL BSA) 3 mls of 500 (ug/mL BSA) 3 mls of 250 (ug/mL BSA) 3 mLs of water (Opg/mL BSA) 3mLs of dialyzed milk solution (previous exp) 3 mls 5 3 mLs 6 3 mls 7 15 mls dialyzed milk solution + 15 mLs water 3 mLs 10.72 10.35 8 3 mls of BSA of unknown concentrate 3 mLs FIGURE 5.4 1. Calculate the concentration of protein in test tubes 1-5. 2. Record the absorbance of the BSA unknown: Concentration of the BSA unknown: 3. You need to prepare a liter of solution with a 300 mg/mL concentration from a stock containing 2000 mg/mL. Calculate the volumes of water and stock solution needed. Exercise 5 Quantitative Analysis of Proteins 51 4. Record the absorbance of your dialyzed milk sample: Concentration of protein in dialyzed milk extract: What is the mass (in mg) of the protein (assuming a volume of 10 mLs)? 5. Submit your standard curve with this worksheet