I need help with 4, 5, and 6 please. Will rate. thank you!

1. Learn the structures, three letter and single letter codes for all 20 amino acids. Draw by hand their structures at pH7.0. 2. Draw the structure of a peptide bond with the bond angles and lengths Lecture 1 slide 9. 3 Draw the pH titration curve for glutamic acid and report the pKa values. Draw each structure. 4. Draw the structure of the pentapeptide, ERNFW, at pH1.5,7.0 and 12.5. 5. Using Ellman's reagent, find the number of cysteines in a protein that has a concentration of 20.0mg/ml solution with a molecular weight of 45000Da. Data- 0.2 ml of the protein was reacted with the reagent and diluted to 10ml and the absorbance at 412nm was 0.773A. Hint, first find the moles of protein used for the experiment, then calculate the moles of Ellman's reagent that reacted. 6. Using the extinction coefficient "generators" given in tive lecture, calculate the Molar extinction coefficient for the three proteins shown below that contains various numbers of tyrosine and tryptophan. Calculate the molecular weight from the residue weights (lecture 3 slide 2). You can use excel to sum the residue weights (attach work sheet to home work.) Report the sequence, the extinction coefficient and the absorbance for a 10 mg/ml solution of each protein. 1) ASDRYWVLIS YYSTQVMCTY WFYGGAAMPK MLAGGDGFLL 2) AWADWTYSTY YWFLLIKWIL WTPDNEQKRY YWLAILVTRE 3) GAATHGRPEE PRODNGGATH KRDCLILLMA THRDILACIE What is the problem with sequence \# 3 ? What would you do to obtain the required information? 1. Learn the structures, three letter and single letter codes for all 20 amino acids. Draw by hand their structures at pH7.0. 2. Draw the structure of a peptide bond with the bond angles and lengths Lecture 1 slide 9. 3 Draw the pH titration curve for glutamic acid and report the pKa values. Draw each structure. 4. Draw the structure of the pentapeptide, ERNFW, at pH1.5,7.0 and 12.5. 5. Using Ellman's reagent, find the number of cysteines in a protein that has a concentration of 20.0mg/ml solution with a molecular weight of 45000Da. Data- 0.2 ml of the protein was reacted with the reagent and diluted to 10ml and the absorbance at 412nm was 0.773A. Hint, first find the moles of protein used for the experiment, then calculate the moles of Ellman's reagent that reacted. 6. Using the extinction coefficient "generators" given in tive lecture, calculate the Molar extinction coefficient for the three proteins shown below that contains various numbers of tyrosine and tryptophan. Calculate the molecular weight from the residue weights (lecture 3 slide 2). You can use excel to sum the residue weights (attach work sheet to home work.) Report the sequence, the extinction coefficient and the absorbance for a 10 mg/ml solution of each protein. 1) ASDRYWVLIS YYSTQVMCTY WFYGGAAMPK MLAGGDGFLL 2) AWADWTYSTY YWFLLIKWIL WTPDNEQKRY YWLAILVTRE 3) GAATHGRPEE PRODNGGATH KRDCLILLMA THRDILACIE What is the problem with sequence \# 3 ? What would you do to obtain the required information