Question: I need help with the quantitative results and the unknown sample Virtual UV-VIS sspectrophotometer Activity #1 Activ. #2 2A Activ. #3 Aux Instructions CC OOO
I need help with the quantitative results and the unknown sample
Virtual UV-VIS sspectrophotometer Activity #1 Activ. #2 2A Activ. #3 Aux Instructions CC OOO DY NO SA A595 = 0.000 .188 .358 Qualitative analysis of results: Observe the relation between colour in the cuvettes and the absorbance. When comparing the measurements, can you observe any dependence between absorbance and the higher or lower concentration of proteins in the cuvette? Quantitative analysis of results: Calculate the concentration of proteins in the final mixture in each cuvette, knowing that the concentration in the bottle is 800 mg/l. Prepare a table with the data and draw a graph plotting A versus C. How would you describe what you are observing? cuvette A595 (mg/) 1 .000 A595 2 .188 3 .358 Cprot (mg/l) 1 ml of an unknown sample is mixed with Bradford's reagent, for a total volumen of 3 ml. Calculate the protein concentration in the original sample if the assay has rendered a value A595 = 0.563 Write your result: c = mg/l and check if it is correct. incorrect
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