(i) Using the absorbance vs time values provided. Calculate vo at each substrate concentration

(ii) Provide a lineweaver burk plot and answer the following questions (a) what type of inhibition is exhibited (b) What is Km and Vmax for the inhibited reaction

NB use graph paper

The system investigated in problem 7.2 was investigated again under identical conditions but in the presence of oxaloacetate initial concentration 2.0 mmol l"' in each case). These results were obtained: Absorbance (at 340 nm) at time 1 (minutes) [NAD) (mmol !!) 0.5 1.0 1.5 2.0 2.5 3.0 1.5 2.0 2.5 3.33 5.0 10.0 0.026 0.042 0.058 0.074 0.088 0.102 0.028 0.045 0.062 0.080 0.096 0.112 0.029 0.047 0.066 0.084 0.102 0.117 0.030 0.050 0.070 0.090 0.108 0,123 0.032 0.053 0.074 0.097 0.115 0.130 0.033 0.057 0.080 0.103 0.124 0.140