(i) Using the Absorbance/time values provided for each substrate concentration (NAD+), calculate the initial velocity (vo) of the reaction at each substrate concentration. (ii) Calculate Km and Vmax. Draw a graph that shows how the kinetic parameters were determined. Hint: use the vo values obtained in (i) and the [NAD+] provided. Construct a lineweaver burk plot and show how Km and Vmax were calculated. Malate dehydrogenase catalyses the reaction: (S)-malate + NAD oxaloacetate + NADH + H* The rate of the forward reaction was investigated in the presence of saturating concentrations of malate and a fixed concentration of enzyme. The following results were obtained: Absorbance (at 340 nm) at time t (minutes) [NAD') (mmol/') - 0.5 1.0 1.5 2.0 2.5 3.0 1.5 2.0 2.5 3.33 5.0 10.0 0.033 0.056 0.079 0.102 0.122 0.138 0.036 0.063 0.089 0.116 0.138 0.154 0.040 0.069 0.099 0.128 0.150 0.168 0.043 0.075 0.108 0.140 0.163 0.175 0.047 0.084 0.121 0.158 0.177 0.184 0.052 0.095 0.137 0.180 0.192 0.200