If you do NOT include the first residues aligned in the subject and query, what is the identity of the first residue mismatch in the subject?

What is the identity of the first mismatch in the query in this alignment?

Color key for alignment scores 40-50 50-80 80-200 > 200 50 100 150 200 250 Dot Matrix View Descriptions Sequences producing significant alignments: Select: All None Selected:0 Aligments Max Total Query E score score cover value Description Ident Accession 406 405 98% 46-146 73% CAA53209.1 Alignments Descriptions ribulose-1,5-biephoephate carboxylase/oxygenase large subunit (chloroplast) [Picea abies] Sequence ID: CAA53209.1 Length: 475 Number of Matches:1 Range 1: 206 to 466 GenPopt Craphics 406 bits 1043) 4e-146 Com Query INSO Next Match Prvous Match Expect Method ional matrix adjust. 19 1(83%) 0/26110%). GAPI 0 Query 1 ANT.DRIPK1tGTmrRVt.AK TARZSGGI 120 EERVLAR IR+ LLEIE SEGDE 445 8b1at 446 ENCKNSEELAANCEIMKBIK 166 Questions/comm Color key for alignment scores 40-50 50-80 80-200 > 200 50 100 150 200 250 Dot Matrix View Descriptions Sequences producing significant alignments: Select: All None Selected:0 Aligments Max Total Query E score score cover value Description Ident Accession 406 405 98% 46-146 73% CAA53209.1 Alignments Descriptions ribulose-1,5-biephoephate carboxylase/oxygenase large subunit (chloroplast) [Picea abies] Sequence ID: CAA53209.1 Length: 475 Number of Matches:1 Range 1: 206 to 466 GenPopt Craphics 406 bits 1043) 4e-146 Com Query INSO Next Match Prvous Match Expect Method ional matrix adjust. 19 1(83%) 0/26110%). GAPI 0 Query 1 ANT.DRIPK1tGTmrRVt.AK TARZSGGI 120 EERVLAR IR+ LLEIE SEGDE 445 8b1at 446 ENCKNSEELAANCEIMKBIK 166 Questions/comm