In a recombinant protein expression process the host microorganism, Pichia pastoris needs to be separated from the
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In a recombinant protein expression process the host microorganism, Pichia pastoris needs to be separated from the culture supernatant after production. A pilot scale bioreactor with a working volume of 200 liters is used for this process and the final biomass concentration is 270 g/L. Considering the separation should be finished in 2 hour. Calculate the sigma factor of a continuous disc stack centrifuge that can fulfil this task assuming density of the microorganism is 1,2 g/L, average size is 3μm (round shape) density of the supernatant is 1,05g/L, viscosity is 5 times the viscosity of water at 25 C.
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