In an enzymatic reaction, A-6x10' min, Ea= 1.1x10 cal/gmol, for enzyme activation, and Ad=4x1046 dk', E-7x104...
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In an enzymatic reaction, A-6x10' min, Ea= 1.1x10 cal/gmol, for enzyme activation, and Ad=4x1046 dk', E-7x104 cal/gmol for deactivation. a) Assuming that the thermal denaturation of the enzyme complies with the 1st order kinetics, graphically show the variation of the maximum reaction rate with temperature in the temperature range of 5 °C -65 °C, taking the concentration Eo = 1 g/L and the of the enzyme for 1 minute and your comments. b) If the same enzyme was kept at 50 °C for 20 minutes, find the decrease in the maximum speed as%. Im= k2 Eo(T,1) Eo(T.1) = [Eo] ekd t exposure time In an enzymatic reaction, A-6x10' min, Ea= 1.1x10 cal/gmol, for enzyme activation, and Ad=4x1046 dk', E-7x104 cal/gmol for deactivation. a) Assuming that the thermal denaturation of the enzyme complies with the 1st order kinetics, graphically show the variation of the maximum reaction rate with temperature in the temperature range of 5 °C -65 °C, taking the concentration Eo = 1 g/L and the of the enzyme for 1 minute and your comments. b) If the same enzyme was kept at 50 °C for 20 minutes, find the decrease in the maximum speed as%. Im= k2 Eo(T,1) Eo(T.1) = [Eo] ekd t exposure time
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Probability and Statistics
ISBN: 978-0321500465
4th edition
Authors: Morris H. DeGroot, Mark J. Schervish
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