In order to measure the enzyme activity and the initial rate of reaction, 5 ml of cellobiose
Question:
In order to measure the enzyme activity and the initial rate of reaction, 5 ml of cellobiose (10 *10^-6 mol/ml) and 44 ml of buffer solution were placed in a stirred vessel. The reaction was initiated by adding 1 ml of enzyme (beta-glucosidase) solution which contained 0.1 mg of protein per ml. At 1, 5, 10, 15, and 30 minutes, 0.1 ml of sample was removed from the reaction mixture and its glucose content was measured. The results were as follows:
What is the activity of the beta-glucosidase in units/ml of enzyme solution and in units/mg protein? A unit is defined as the enzyme activity which can produce 1 mumol of product per minute.
What is the initial rate of reaction?
Time (min) | Glucose concentration (?mol/ml) |
1 | 0.05 |
5 | 0.23 |
10 | 0.38 |
15 | 0.52 |
30 | 1.03 |
Mathematical Statistics with Applications in R
ISBN: 978-0124171138
2nd edition
Authors: Chris P. Tsokos, K.M. Ramachandran