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Quantitative Chemical Analysis Harris | Lucy TENTHETICS presented by Macmillan Learning In monolithic columns 24 the stationary phase is a single porous piece of silicia or polymer filling the entire column and synthesized within the column from liquid precursors. Monolithic columns offer similar plate height to HPLC particles, but with less resistance to flow. Therefore, faster flow or longer columns can be used. The figure shows separation of isotopic molecules on a long monolithic column. Packed columns have too much resistance to flow to be made so long. 193.3 min D 194.3 min D 188.1 D Dmin > D D Nie Ar- 1.01 min W12 1.01 min 185 190 195 Time (min) Separation of isotopic molecules on a 440-cm-long monolithic silica column cluted with CH,CN/H,O (30:70 vol/vol) at 30C [Data from K. Miyamoto, T. Hara, H. Kobayashi, H. Morisaka, D. Tokuda, K. Horie, K Koduki, S. Makino, O. Noe, C. Yang, T. Kawabe, T. Ikegami, H. Takubo, Y. Ishihama, and N Tanaka, "High-Elciency Liquid Chromatographie Separation Utilising Long Monolithic Silica Capillary Columns, Anal Question outer Quantitat Y. Ishihama, and N. Tanaka, "High-Efficiency Liquid Chromatographie Separation Utilizing Long Monolithic Silica Capillary Columns, Anal Chem. 2008, 80,8741.) Unretained thiourea is cluted in 41.7 min. Find the linear velocity we (mm/s). mm Find the retention factor k for CD k Find the plate number N and plate height for CD N = ke Find the plate number N and plate height for CD N = H = im Assuming that the peak widths for C, H,D and C, H, are the same as that of C, Dfind the resolution between C, H,D and CH Resolution Retention times for CHD and CH are 193.3 and 194,3 min, respectively. Find the separation factor (m) between C, H, D and CH Out Source Quantitative Che