Let's say you have nucleotide sequence BLAST database containing 10,000,000 sequences averaging 100 nucleotides in length. You are interested in BLASTing another sequence that is 20-nt in length. What E-value would you expect if the bit-score (S' in the notes) of the top hit is 5? What E-value would you expect if the bit-score (S' in the notes) of the top hit is 35? Hint: You can raise a number to a power in python with the double-asterisk operator. So 2-100 would be written in python as:

>>> 2**-100 7.888609052210118e-31

Let's say you have nucleotide sequence BLAST database containing 10,000,000 sequences averaging 100 nucleotides in length. You are interested in BLASTing another sequence that is 20-nt in length. What E-value would you expect if the bit-score (S' in the notes) of the top hit is 5? What E-value would you expect if the bit-score (S' in the notes) of the top hit is 35? Hint: You can raise a number to a power in python with the double-asterisk operator. So 2-100 would be written in python as: >>> 2**-100 7.888609052210118e-31 Let's say you have nucleotide sequence BLAST database containing 10,000,000 sequences averaging 100 nucleotides in length. You are interested in BLASTing another sequence that is 20-nt in length. What E-value would you expect if the bit-score (S' in the notes) of the top hit is 5? What E-value would you expect if the bit-score (S' in the notes) of the top hit is 35? Hint: You can raise a number to a power in python with the double-asterisk operator. So 2-100 would be written in python as: >>> 2**-100 7.888609052210118e-31