Tris buffer uses the following equilibrium to make buffers near pH 7: OH OH . OH...

 

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Tris buffer uses the following equilibrium to make buffers near pH 7: OH OH НО. OH HO OH + H NH₂ NH3 The pKb of Tris (C(CH₂OH)3NH2) is 5.92, so the pKa of Trish is 8.08. a. What is the pH of a solution with 10 mM Tris and 10 mM TrisH", assuming ideal solutions? b. What is the pH of a solution with 10 mM Tris and 10 mM TrisH, with 100 mM NaC1 present? ATP binds to magnesium ions (Mg), as shown below: ATP4- (aq) + Mg²+ (aq) Mg* ATP²- (aq) a) AG is -16 kJ mol. In a solution with 0.25 M CaCl and 0.010 M MgCl: what is the ratio of MgATP to uncomplexed ATP at equilibrium if you assume ideal solution conditions at 298 K? b) AG is -16 kJ mol. In a solution with 0.25 M CaCl₂ and 0.010 M MgCl₂, what is the ratio of MgATP to uncomplexed ATP at equilibrium if you do not assume ideal solution conditions at 298 K? c) Conceptually, why does the high salt concentration decrease magnesium complexation to ATP? Tris buffer uses the following equilibrium to make buffers near pH 7: OH OH НО. OH HO OH + H NH₂ NH3 The pKb of Tris (C(CH₂OH)3NH2) is 5.92, so the pKa of Trish is 8.08. a. What is the pH of a solution with 10 mM Tris and 10 mM TrisH", assuming ideal solutions? b. What is the pH of a solution with 10 mM Tris and 10 mM TrisH, with 100 mM NaC1 present? ATP binds to magnesium ions (Mg), as shown below: ATP4- (aq) + Mg²+ (aq) Mg* ATP²- (aq) a) AG is -16 kJ mol. In a solution with 0.25 M CaCl and 0.010 M MgCl: what is the ratio of MgATP to uncomplexed ATP at equilibrium if you assume ideal solution conditions at 298 K? b) AG is -16 kJ mol. In a solution with 0.25 M CaCl₂ and 0.010 M MgCl₂, what is the ratio of MgATP to uncomplexed ATP at equilibrium if you do not assume ideal solution conditions at 298 K? c) Conceptually, why does the high salt concentration decrease magnesium complexation to ATP?