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15 points . ' hy M: 5 | performed their critical experiment no particular reason why Matt Meselson and Frank Stah ritice By cwieting hein heavy to light nitrogen, They would have come to the same conclusion if they went from light to heavy nitrogen. In fact, they did it both wayswith some minor difficulties that are discussed in this brief video clip (https: www.dnalc.org/view/16456-Video-20-Frank-Stahl-clip-3.html). Part A) What would the cesium chloride gradient tubes look like following centrifugation for this light-to- heavy nitrogen media shift experiment using the three models of conservative, semi-conservative, and dispersive DNA replication (where the bacteria grow to two generations following the media shift)? Relative location of the bands and the intensity of the bands are the critical considerations in this question. -| Part B) An unusual species (Madeupicus replicatium) isolated from hot springs in the Jellystone Park has been shown to replicate its DNA by a conservative mechanism at 25C and semiconservative mechanism at 95C. You repeat the eselson-Stah! experiment, but you vary the nitrogen and temperature. First, you grow all the bacteria in light nitrogen media 25C. Next, you centrifuge them and switch to heavy nitrogen while changing the temperature 95C. Finally, you centrifuge the bacterial and switch back to light nitrogen and a temperature of 25C. You take a sample in the pre-media shift, after one generation in heavy media, and after another generation in light media at 25C. media | temperature | Pre-media shift 14N Za | First generation post-shift ISN 95C | Second generation post-shift 14N 2570. c. What would the cesium chloride gradient tubes look like following centrifugation for this experiment? Relative location of the bands and the intensity of the bands are the critical considerations in this question. 2. Note that all parts of this question refer to the DNA sequence given below. DNA: 5' -GTCTTACTTATCAGGCATAAATCCG -3' 3\" -CAGAATGAATAGTCCGTATTTAGGC -5/ @) If the origin of replication were to the right of the DNA, would the t ; op or bottom strand template for leading strand synthesis? ee b) Which of the two DNA strands present above will be heavie i vier and give your r St give your rationale for the