MCB 2000 Problem Set 1B Spring 2023 ANOTE YOU MUST SHOW YOUR WORK FOR FULL CREDIT! "Answer Questions on a Separate Sheet of Paper! 1. (5 points) How many micrograms of solid NaCl (Formula Weight: 58.44 g/mole) are required to prepare 250mL of a 50mM solution? 2. (15 points) If you dissolve 45mg glucose (Formula Weight: 180.16g/mole ) in 61mL of water (all answers must be presented in scientific notation format): a. What is the concentration of glucose in mM and M ? b. If you take 10mL of this solution and dilute it to 500mL, what is the concentration of the glucose in the resulting solution in M ? c. How many nanomoles of glucose are present in 10mL of the diluted solution? 3. (5 points) Convert the concentration below to mM and M : a. 40mg of sucrose (Formula Weight: 342.30g mole) per 156mL. 4. (10 points) What molarity of HCl stock solution is needed so that 1.25mL of this stock solution diluted to 0.050L will yield 50mM ? 5. (10 points) You have to make 50mL of 0.05MHCl from a 10MHCl stock solution, but the only measuring devices available are 100mL graduated cylinders and 10mL serological pipets. How could you accurately make the dilute HCl solution? 6. (25 points) You have a 0.25M stock solution of NaCl (Formula Weight: 58.4g/mole ), a 0.05M stock solution of glucose (Formula Weight; 180.2g/mole), and a bottle of solid Tris base (Formula Weight: 121.1g/mole ). How would prepare (be specific) 500mL of a single solution containing 250mM Tris, 5mM glucose, and 25mMNaCl. 7. (10 points) Clearly describe how you would prepare 15mL of a 0.50M stock solution of potassium chloride (Formula Weight: 74.55g/ mole). Using this stock solution, describe how you would prepare 50mL of the following dilutions: 2.5mM and 5000M 8. (10 points) How many micrograms of MgCl2 (Formula Weight: 95.211g/mole ) are contained in 25mL of 150mMMgCl (answer must be presented in scientifie notation format)? 9. (10 points) What is the molar concentration of potassium chloride (Formula Weight: 74.55 g/ mole) in a 5%(w/v) solution? 5. (10 points) You have to make 50mL of 0.05MHCl from a 10MHCl stock solution, but the only. measuring devices available are 100mL graduated cylinders and 10mL serological pipets. How could you accurately make the dilute HCl solution? 6. (25 points) You have a 0.25M stock solution of NaCl (Formula Weight: 58.4g/mole ), a 0.05M stock solution of glucose (Formula Weight; 180.2g/mole ), and a bottle of solid Tris base (Formula. Weight: 121.1g/mole ). How would prepare (be specific) 500mL of a single solution containing 250mM Tris, 5mM glucose, and 25mMNaCl. 7. (10 points) Clearly describe how you would prepare 15mL of a 0.50M stock solution of potassium chloride (Formula Weight: 74.55g/mole ). Using this stock solution, describe how you would prepare 50mL of the following dilutions: 2.5mM and 5000M 8. (10 points) How many micrograms of MgCl2 (Formula Weight: 95.211g/mole ) are contained in 25mL of 150mMMgCl (answer must be presented in scientific notation format)? 9. (10 points) What is the molar concentration of potassium chloride (Formula Weight: 74.55 g/mole) in a 5%(w/v) solution