Microplate Schematic: 2. A BCA assay is a commonly used colorimetric assay for accurately determining protein concentration (direct measurement at A280 is not accurate). This assay consists of Bovine Serum Albumin (BSA) at 2 mg/ml (this is the stock solution) which is a protein standard and Colour Reagent which produces more purple colour when in the presence of more protein. The Colour Reagent is added directly to each standard and unknown sample before being read at 562 nm on a plate reader. The assay has a detection range of 20-2000 ug/ml a. Fill in the following table with the volumes of H20 and stock BSA required for each standard (dilutions are made with H20) such that the final volume adds up to the volume indicated in the 4" column (9 marks). Tube Volume of H20 Volume of BSA Final Volume in Final BSA Concentration Tube (UL) (BSA+H20) (ug/ml) A ul of stock 300 2000 B UL of stock 500 1500 C HL of stock 650 1000 D UL of tube B dilution 350 750 E HL AL of tube C dilution 650 500 F ULL HL of tube E dilution 650 250 G UL of tube F dilution 650 125 H UL UL of tube G dilution 500 25 ULL HL of stock 400 0 = Blank On the next page you will find a schematic of how a microplate was loaded with the standards indicated above and three protein samples of unknown concentration. There is also a figure of the standard curve produced and a chart of the absorbance values of each standard and protein sample which matches the order of the microplate schematic