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4. The following data are for a liquid chromatographic column: A chromatogram of a mixture of species A. B, C, and D provided the following data: Calculate (a) the number of plates from each peak. (b) the mean and the standard deviation for N. (c) the plate height for the column. From the data above, calculate for A,B,C, and D : (d) the retention factor. (c) the distribution constant. From the data above, calculate for species B and C : (f) the resolution. (g) the selectivity factor. (h) the length of column necessary to separate the two species with a resolution of 1.5. (i) the time required to scparate the two species on the column in part (b)