Need help with just the POST LAB. I've attached the pre-lab answers as well, because some of the answers from the pre-lab are needed to answer the post lab. Thank

you so much.

PRE-LAB QUESTIONS 1. Acetylcholine is sold as acetylcholine chloride. Looking at the structures given in the Background, write the formula for the anion and the cation of this salt. Amon:ClCadion:CH3C(O)OCH2CH2N+(CH3)3 2. The molecular weight of acetylcholine chloride is 181.7. A stock solution of 1.817g acetylcholine in 10.0mL was prepared by the stockroom. What is the concentration of this stock solution in %w/v and in molarity? From this stock solution of acetylcholine chloride, 0.40mL was added to the sample tube bringing the total volume in the sample tube to 3.0mL. What is the concentration of the acetylcholine chloride in the sample tube in g/mL and in molarity? a) 1.817g/10.0ml(x.1007=18.11%w/v 6) 1.8179((181.7g(mpl)10mL(n)/1000ml=1.000M) c) 0.40mL. 0.18.11w/v/1.0ml=2.42%w/v d) 0.40mb(x)1.0M/2.0mL=0.04HM/M 3. Acetylcholine esterase, 3.0mg, was dissolved in 10.0mL solution. From this solution, 0.60mL was added to the sample tube, bringing the volume to a total of 3.0mL. How many mg of acetylcholine esterase did you have in the sample tube? 0.h0 mef/WL 8 o. 60 mLL =0.16nss 4. The commercial sample of carboxyl esterase contained 15.0mg of enzyme in 0.30mL. suspension. In your experiment, you add 0.090mL of this suspension to the sample tube that contains a total volume of 3.0mL. How many mg of carboxyl esterase enzyn do you have in the sample tube? b. carboxyl esterase c. You have calculated the amount of acetylcholine esterase that you had in the sample tube (Pre-Lab Question no. 3). Use this number to calculate the activity of this enzyme toward acetylcholine in absorbence/min/mg enzyme. d. You have calculated the amount of carboxyl esterase that you had in the sample tube (Pre-Lab Question no. 4). Use this number to calculate the activity of this enzyme toward acetylcholine in absorbence/min/mg enzyme. e. Which enzyme catalyzes the hydrolysis of acetyleholine faster? How many times faster? 2. Calculate the enzyme activities of o-nitrophenyl acetate as a substrate for both enzymes. Using the change in absorbence between 30sec. and 2min. in the reaction, calculate the rate of reaction toward o-nitrophenyl acetate in absorbence/min. for a. acetylcholine esterase b. carboxyl esterase c. You have calculated the amount of acetylcholine esterase in your sample tube (Pre-Lab Question no. 3). Use this number to calculate the activity of this enzyme toward o-nitrophenyl acetate in absorbence/min/mg enzyme. d. You have calculated the amount of carboxyl esterase in your sample tube (Pre-Lab Question no. 4). Use this number to calculate the activity of this enzyme toward o-nitrophenyl acetate in absorbence/min/mg enzyme. e. Which enzyme catalyzes the hydrolysis of o-nitrophenyl faster? How many times faster