Note: Solve Q-2 only

Q-1.

Consider a well-mixed 4000 L bioreactor operating at 37 C. In order to maintain steady state

temperature due to heat loss through the vessel, a stainless-steel jacket is required and

operated at an inlet temperature of 60 C, outlet at 52 C and flowrate of 10 gpm (gallons per

minute). Assume no heat generated from cell growth. Answer the following questions based

on this information;

a. How much heat (KJ) can this jacket remove from the bioreactor?

b. A technician observes that the jacket pipping has a slight leak upstream of the bioreactor

(leak is located on pipping that is before the actual bioreactor jacket). A bucket is used to

identify a volumetric leak rate of 5 LPM (liters per minute). If this is correct and there are no

further leaks, answer the following;

i. Draw the process flow diagram required and define your system boundary

ii. What is the new outlet temperature (C) that we should observe on the outlet

of the jacket?

Q-2

Consider the well-mixed 4000L bioreactor from Question 1. The bioreactor is operated in

perfusion mode with CHO cells at a density of 1E9 cells/mL. A small-scale experiment

shows that the glucose demand is 0.02 ng/cell. For CHO cells, assume that the enthalpy of

reaction (Hrxn) due to cellular metabolism is ~ -2760 KJ/mol-glucose. Answer the

following questions:

a. How much heat (kJ) will be generated from the reactor if it is well-insulated?

b. Assume the bioreactor from Question 1 is not insulated and does not have a leaking

jacket (i.e, heat loss similar to part 1a). The inlet of the jacket is still maintained at 60 C and

a flowrate of 10 GPM. What is the new outlet temperature (C) from the jacket if we now

consider cellular growth heat generation and the heat loss from 1a?