Question: Part 1. For your sample, you have 2 organisms isolated: 1. Staphyloccus aureus - you have a Gram Positive cocci in clusters with positive growth
Part 1.
For your sample, you have 2 organisms isolated:
1. Staphyloccus aureus - you have a Gram Positive cocci in clusters with positive growth on MSA with yellow colonies (means ferments Mannitol)
2. Enterobacteriaceae NonLactose Fermenters - you have Gram Negative Bacilli with positive growth on MacConkey agar with colorless colonies (means NonLactose Fermenters), examples of Nonlactose Fermenters were Proteus, Salmonella, Shigella, Pseudomonas, Yersinia. You may differentiate them with their IMVIC reactions,
Part 2.
1. Work up for Staphylococcus spp. After Gram Staining, you can do Catalase Test, If positive, it is Staphylococus spp. , then do Coagulase test, if Positive it is S. aurues, if negative, these are CONS, you can procede with Novobiocin sensitivity test then. (Check out the table picture Ill send at the explanation)
2. Work up the Enterobacteriacea, you can have the Oxidase test first then the IMViC test (I for Indole test, M for Methyl Red test, V for Voges-Proskauer test, and C for Citrate test). After that, you can look at the table results for IMVIC reactions, which is present on the internet. Can't upload the image since they have copyrights here. But nowadays, this test is only done at schools for learning purposes, the one that is being used now at the lab is Crystal ID, wherein it's automated.
Can I have a plan to test the organisms?
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