Part 3

We will now simulate gene expression using a random number generator.Go to www.random.org. Generate a random number for between 1 and 10. Do this for each below and record these numbers:

First random number:__________6____________________(use this number for the level of gene expression of clrD associated with pmtrA on your bar chart)

Second random number:___________4________________ (use this number level of gene expression of clrD associated with pmtrBon your bar chart)

Part 4

Using the numbers above, draw a bar chart below.

1. Give your chart an appropriate title.
2. What is your independent variable?
3. Give the x-axis an appropriate label.
4. What is your dependent variable?
5. Give the y-axis an appropriate label.
6. Draw and label bars for the levels of gene expression using the numbers generated in Part 3.
7. What would you expect a level of GFP expression to look like for a negative control? What you would expect the level of GFP expression to look like for a positive control? Draw and label bars for what you would expect for your controls.
8. Write a summary statement about your results below.