Partial hydrolysis of a protein yielded a number of polypeptides. Deduce the sequence of amino acids in

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Question:

Partial hydrolysis of a protein yielded a number of polypeptides. Deduce the sequence of amino acids in this polypeptide from the following information with a propore justification:

(a) Complete acid hydrolysis yielded ala+ arg+2 ser+ lys+ phe+ met+ trp+ pro.

(b) Treatment with fluorodinitrobenzene (FDNB) followed by complete acid hydrolysis yielded dinitrophenylalanine (DNP-ala) and E-dinitrophenyllysine (e-DNP-lys) as the only DNP derivatives

(c) Carboxypaptidase did not release a c-terminal amino acid. This enzyme catalyzes the hydrolysis of the carboxyl-terminal residue of a polypeptide unless the residue is Pro or, for some reason, does not contain a free carboxyl group

(d) Treatment with cyanogens bromide yielded two peptides. One contained ser + trp+ pro. The other contained all the remaining amino acids (including the second ser).

(e) Treatment with chymotrypsin yielded three peptides. One contained only ser + pro. Another contained only met + trp. The third contained phe + lys + ser+ arg + ala.

(f) Treatment with trypsin yielded three peptides. One contained only ala+ arg. Another contained only lys + ser. The third contained phe + trp + met+ ser + pro.