Question: pH needs to be 7.1 Make me a phosphate buffer with a given p H value (see below) so I can study some blood proteins.

pH needs to be 7.1

Make me a phosphate buffer with a given p H value (see below) so I can study some blood proteins. The concentration of ions in blood (it's ionic strength) is in the neighbourhood of 150mM.1 Make me enough so that I can have at least 1000 samples of 2mL each. Data: pKa values for phosphoric acid, H3PO4, are 2.1,7.2 and 12.3 You have three bottles of crystalline solids: NaH2PO4, mw 120g/mole;Na2HPO4, mw 142g/mole;Na3PO4, mw 164g/mole. I spent the big bucks on anhydrous salts for you. As you do your calculations please consider what properties are desirable in a biological buffer

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