please answer a b c d and e. thank you!!

Patto. Diakis 8. We ar-40osto transter the dissolved protein solution to a dialysis tube/sack. Record your sample number Thom the arange dialysis sack clip. =57 dialesis sack will be kept in a container filied with 20mM phosphate buffer pH 6.5 until the next step of protein purification that you will apply ion-exchange chromatography to further increase the purity of myoglobin (Lab 44). while SAMPLES A. B. and Care kept froten for the analysis of orotein concentrations. (Lab a5) and their protein contents ( Lab#6). 0.3. Wash and return the 50-mL centrifuge tube and cap to the rack at the front of the lab. Data Analysis Questions (remember to cite any used references) 2. What are in the precipitates after the first centrifugation? [1.0] b. When adding ammonium sulfate the first time you are bringing the solution to 60% ammonium sulfate saturation. At this point, what do you predict is in the supernatant? Why? What do you predict is in the pellet, and why? [2.0} c. What is contained in the peliet and supernatant after saiting out (up to 100% saturation)? Why? [2.0] - d. What is the purpose of diatysis and how is this important for the success of the next step in the purification process, ion-exchange chromatography? [1. 0) e. What is in the structure of myoglobin that reacts with potassiam ferricyanide(iu) to cause the color change? What are the purplish-red and the brown forms of myoglobin? Since myoglobin has oxygen binding property, can these the purplish-red and the brown forms of myoglobin still bind oxygen? [2.0] Assignment Requirements: Answers to Data Analysis Questions (typed) and the Qata Sheet are due at the beginning of the next lab sesslon! (9.0 marks) Upon completion of the experiment, make sure to set the micropipette to its maximum limit and place vertically in the pipet holder. (A) The wery end afier all paits complese = tinal lagereport just clata anaiuare pring to lob call frintere ou frinter Frill