Question: Please answer questions 2 & 3 for GFP determination ! 1. Generate a figure for the 300 - 600 nm scan. 2. Calculate the concentration

Please answer questions 2 & 3 for GFP determination !

1. Generate a figure for the 300 - 600 nm scan.

2. Calculate the concentration using the extinction coefficient (56,000 Cm^-1 M^-1) and then calculate the total amount of GFP in the original, undiluted, crude sample. (dilution 1:10 with final volume of 200uL 3. Calculate the relative purity (R.P.) of your crude extract supernatant.

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemical Engineering Questions!

Please answer questions 2 & 3 for GFP determination! 1. Generate a figure for the 300 - 600 nm scan. 2. Calculate the concentration using the extinction coefficient (56,000 Cm^-1 M^-1) and then...

An Investigation into obesity rates Researchers have been interested in studying the obesity trends worldwide over the past 25 years. In 2014 the World Health Organization (WHO) declared there were....

Please quality check my solutions for this advanced heat transfer problem. Show all work. Thank you. All original documents are located at the bottom. 3.9 COMPACT HEAT EXCHANGERS 3.9.10-1 3.9.10...

ID Grip Strength (X) (X-Mean)2 98.5 3.4596 Histogram of Grip Strength in male EXSC 415 students $5.5 1.2996 10 95.0 2.6896 98.0 1.8496 126.5 891.6196 90.0 44.0896 114.0 301.3696 108.0 129.0496 124.0...

Please evaluate the quality of the design (answer questions within 1-4 on the gas turbine recuperator rating problem). Please answer all questions asked. Show all calculations. I have provided...

Attached is Accounting assignment along side recommended readings to answer certain questions. Thank you Assignment 1 Problem 1 15 points Reading - W. L. Ferrara, Cost/Management Accounting: The 21st...

Set Student Name: 1. Describe the relationship between two variables that have a correlation coefficient value: a. Near -1 b. Near 0 c. Near 1 2. Data was collected where a weightlifter was asked to...

I have attached the case study and the rubric for this. can I get help here to organzie an answer? CATERPILLAR, INC: THE IMPACT OF DECISION BIASES AND RISK ON CAPITAL BUDGETING Timothy D. West...

Instuctor's Annotated Edition TENTH EDITION Understandable Statistics Concepts and Methods Charles Henry Brase Regis University Corrinne Pellillo Brase Arapahoe Community College Australia Brazil...

Can anyone help me with 1-3 and number 5 please. PRE-LAB QUESTIONS 1. Refer back to generalized chemical reaction presented in the introduction and write two comparable rate expressions for reactant...

Based on the results from your lab, how might your results differ if you direct the air flow onto the upper surface of the leaf versus the lower surface? Why?

In order for investors to purchase stock in a company, they will require a return of at least a certain amount. The amount investors require depends on which of the following? Group of answer choices...

5. Develop a scenario comparing two PH programs and involving the use of a CBA.

3. Western Europe seems to be uniquely involved with various forms of worker participation. What are some reasons that these worker participation systems have developed so fully there instead of...

1. Evaluate the exchange rates and wage rates shown in Exhibit 14.1 and decide where you should dock your ship for local production. You are the owner of a small North Carolina factory that makes...

6. Which features of Canadas labor relations system would you transfer to the United States?