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Absorbance [Denaturant]M Remark Use the data to determine the free Helpful Equations 0.985 0 100% folded, ymax energy of unfolding in the absence 0.985 0.1 Fraction unfolded: 0.985 0.2 of denaturant (AG,,) for this 0.98 Fu= (Amax-A)/(Amax-Amin) 0.3 0.975 0.4 protein. Fraction folded: 0.97 0.5 0.96 0.6 Note that the denaturation curve Ff = 1 - Fu 0.945 0.8 0.89 goes from high to low absorbance 1 K = Fu / FF 0.76 1.2 in this case. See slides for theory 0.625 1.4 0.51 Free energy of unfolding: 1.6 and calculations. T=298K. 0.375 1.8 AG, =-RTInky 0.325 1.9 0.285 2 Email your answer, two graphs and 0.22 2.1 For finding AGu,w : 0.175 2.2 calculations to Dr. Ding before 11:59 AGy= AGu,w - m*[Urea]! 0.14 2.4 0.12 2.5 pm on September 25th, 2023. 0.115 2.6 Remember UNITS in your answer! 0.11 2.7 0.105 2.8 0.105 2.9