Please explain step by step and clear drawing!

Have a nice day!

PS.

A clarification: You may use the same dipodal linkage for all of the PEG chains to the gold/SiO2 surface. This should simplify your designs.

Protein Immobilization with Orthogonal Reactions 1) You are interested in developing a dual-function biosensor for detection of West Nile viruses and pathogenic bacteria in blood. To achieve this aim, you will use an IgG antibody against the West Nile capsid proteins, and a synthetic peptide (WLBU2) which binds the cell membranes of Gram-negative pathogens. The sensor is to be based on a quartz-crystal microbalance (QCM), which is extremely sensitive to tiny changes in the mass at the sensor surface. Therefore, you will produce a short PEO brush to prevent non-specific adsorption of proteins and cells, and immobilize the recognition elements (-Abs and WLBU2) on longer PEO tethers so that they are presented above the non-fouling brush layer You have decided to engineer the surface architecture like that shown schematically below Black lines represent PEO chains, the green lines are linkages between the surface and PEO the red/blue spirals represent WLBU2 peptides, and the red shapes represent short half-Abs. In order to minimize cross-reactivity with host immune cells, the Fc region of the antibodies should be removed before immobilization. Au or Sio2 Protein Immobilization with Orthogonal Reactions 1) You are interested in developing a dual-function biosensor for detection of West Nile viruses and pathogenic bacteria in blood. To achieve this aim, you will use an IgG antibody against the West Nile capsid proteins, and a synthetic peptide (WLBU2) which binds the cell membranes of Gram-negative pathogens. The sensor is to be based on a quartz-crystal microbalance (QCM), which is extremely sensitive to tiny changes in the mass at the sensor surface. Therefore, you will produce a short PEO brush to prevent non-specific adsorption of proteins and cells, and immobilize the recognition elements (-Abs and WLBU2) on longer PEO tethers so that they are presented above the non-fouling brush layer You have decided to engineer the surface architecture like that shown schematically below Black lines represent PEO chains, the green lines are linkages between the surface and PEO the red/blue spirals represent WLBU2 peptides, and the red shapes represent short half-Abs. In order to minimize cross-reactivity with host immune cells, the Fc region of the antibodies should be removed before immobilization. Au or Sio2