please help me with these

1) (3pts) Include a chromatogram for each of your Retention Time Check samples. Label the peaks with the analyte name and exact retention time. a. You don't need to include the chromatograms for the standards or the unknowns. 2) (4pts) Create an external calibration curve without using your internal standard. a. Use the area under the curve (rather than peak height) of your standards in your plot. b. Perform a least squares analysis (add a trendline) to determine the equation of the linear portion of the calibration curves. Be sure to add the equation and R value for your trendline to your plot. 3) (4pts) Create an external calibration curve using your internal standard. a. Perform a least squares analysis (add a trendline) to determine the equation of the linear portion of the calibration curves. Be sure to add the equation and R value for your trendline to your plot. 4) (10pts) Create a table that includes the following: a. The area under the curve of for the ethanol and chloroform peaks for each of your unknown replicates. Include the average and standard deviation for these samples. b. The signal ratio of the analyte to internal standard for each of the unknown replicates. Include the average and standard deviation for these samples. c. The BAC of the suspect's sample using your external calibration curve without the internal standard. Calculate this for each replicate and then calculate the average and standard deviation. d. The BAC of the suspect's sample using your external calibration curve using the internal standard. Calculate this for each replicate and then calculate the average and standard deviation. e. Include your work for the calculations in c and d. GC Lab 0.08% BAC 0.08/CHCI3 1 2 3 Retention Time Retention Time Peak Area Peak Area Concentration (Ethanol) (CHC13) (Ethanol) (CHCIB) Ratio 1.391 121.6 1.391 1.816 336.8 2503.4 0.12 1.392 1.819 183.4 281) 0.06524 0.10 1.392 1.82 146.9 2809.81 0.05228 0.06 1.388 1.814 77.2 2593.1 0.02977 0.04 1.39 1.816 2683.5 0.02217 0.02 1.389 1.816 25.2 2538.5 0.00993 59.5 4 5 0.07 0.06 0.05 0.04 Ratio of peak areas 0.03 0.02 0.01 0 0 0.02 0.04 0.1 0.12 0.14 0.06 0.08 Concentration 1) (3pts) Include a chromatogram for each of your Retention Time Check samples. Label the peaks with the analyte name and exact retention time. a. You don't need to include the chromatograms for the standards or the unknowns. 2) (4pts) Create an external calibration curve without using your internal standard. a. Use the area under the curve (rather than peak height) of your standards in your plot. b. Perform a least squares analysis (add a trendline) to determine the equation of the linear portion of the calibration curves. Be sure to add the equation and R value for your trendline to your plot. 3) (4pts) Create an external calibration curve using your internal standard. a. Perform a least squares analysis (add a trendline) to determine the equation of the linear portion of the calibration curves. Be sure to add the equation and R value for your trendline to your plot. 4) (10pts) Create a table that includes the following: a. The area under the curve of for the ethanol and chloroform peaks for each of your unknown replicates. Include the average and standard deviation for these samples. b. The signal ratio of the analyte to internal standard for each of the unknown replicates. Include the average and standard deviation for these samples. c. The BAC of the suspect's sample using your external calibration curve without the internal standard. Calculate this for each replicate and then calculate the average and standard deviation. d. The BAC of the suspect's sample using your external calibration curve using the internal standard. Calculate this for each replicate and then calculate the average and standard deviation. e. Include your work for the calculations in c and d. GC Lab 0.08% BAC 0.08/CHCI3 1 2 3 Retention Time Retention Time Peak Area Peak Area Concentration (Ethanol) (CHC13) (Ethanol) (CHCIB) Ratio 1.391 121.6 1.391 1.816 336.8 2503.4 0.12 1.392 1.819 183.4 281) 0.06524 0.10 1.392 1.82 146.9 2809.81 0.05228 0.06 1.388 1.814 77.2 2593.1 0.02977 0.04 1.39 1.816 2683.5 0.02217 0.02 1.389 1.816 25.2 2538.5 0.00993 59.5 4 5 0.07 0.06 0.05 0.04 Ratio of peak areas 0.03 0.02 0.01 0 0 0.02 0.04 0.1 0.12 0.14 0.06 0.08 Concentration