please read and do question part 4.2-4.3

For this exercise, you will use a software suite that is called GALAXY to analyze five RNA-Seq datasets. The datasets are paired-end 50bp reads from adrenal and brain tissues (500Kb region of chromosome 19, chr 19:3000000:3500000). You can find the datasets here: httos: /lusegalaxy orgiulieremy/p/galaxy-rna-seq-analysis exercise The datasets contain the transcriptional profiles in those tissues and our task is to find genes that are differentially expressed (DEG) in the sample conditions versus the control sample. We have 3 programs to perform this task: edgeR, Cuffdiff and DESeq2. You are asked to do this analysis with each and finally report the results. Exercise 4.1. (10 points) Using Cuffdiff from Galaxy, complete the tutorial that is found in the previous link and report the top 100 DEG, their fold-change (or score) and their p-values. Exercise 4.2. (10 points) Perform the same analysis by using EdgeR, which can be found here: http://www.bioconductor.org/packages/release/bioc/html/edgeR html Again report the top 100 DEG based on EdgeR, their fold-change (or score) and their p-values Exercise 4.3 (10 points) Finally, perform the same analysis by running DESeq2: htto:/bioconductor orgipackages/release/biochtmi/DESeq2 html And (as you have guessed) report the top 100 DEG, their fold-change (or score) and their p-values