Question: PLEASE USE MS EXCEL. PLEASE DONT ANSWER NUMBER 9 JUST NUMBER 8 AND 10 8. Determine the values of Km and Vmax for the decarboxylation

PLEASE USE MS EXCEL. PLEASE DONT ANSWER NUMBER 9 JUST NUMBER 8 AND 10

PLEASE USE MS EXCEL. PLEASE DONT ANSWER NUMBER 9 JUST NUMBER 8

AND 10 8. Determine the values of Km and Vmax for the

8. Determine the values of Km and Vmax for the decarboxylation of B-keto acid given the following data. Please show and label your plot. Show your calculations and box your final answers. (S) in mol/IV in mm/min 2.500 0.588 1.000 0.500 0.714 0.417 0.526 0.370 0.250 0.256 9. For the following aspartate reaction in the presence of inhibitor, Km = 0.00065 M. Determine Vmax in both reactions and in the reaction without inhibitor, the Km. Identify whether the inhibition is competitive, non-competitive or uncompetitive. 110 100- 1/ 1/V 00 R 60 1/ 50- 5 20- 10- Forg For F0009 F0008 00001 12000 10. For your answer in #9, explain briefly: a. 1. how I and S bind to the E as shown by the Lineweaver Burk plot. b. the significance of the following obtained values for Km and Vmax. c. effect in slope and x-intercept

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