Post-Lab Assignment Once you have completed the lab, answer the following Discussion Questions below, and place your answers in YOUR CONCLUSIONS SECTION!!! You may answer these questions in numbered order, one-by-one OR in paragraph form. Show any and all calculations where applicable! Discussion Questions: 1. Discuss the identified alteration/mutations made to the GFP sequence and what effect(s) (if any) on the function of GFP they may have had. 2. Based on your expanded understanding of the EGFP protein sequence, its mutations from the GFP sequence, and your new knowledge of the EGFP structure, consider the following: a. If you were to mutate the glutamate-222 residue to an alanine residue, what effect (if any) would there be on the function of EGFP? Post-Lab Assignment Once you have completed the lab, answer the following Discussion Questions below, and place your answers in YOUR CONCLUSIONS SECTION!!! You may answer these questions in numbered order, one-by-one OR in paragraph form. Show any and all calculations where applicable! Discussion Questions: 1. Discuss the identified alteration/mutations made to the GFP sequence and what effect(s) (if any) on the function of GFP they may have had. 2. Based on your expanded understanding of the EGFP protein sequence, its mutations from the GFP sequence, and your new knowledge of the EGFP structure, consider the following: a. If you were to mutate the glutamate-222 residue to an alanine residue, what effect (if any) would there be on the function of EGFP