Pre$-$Lab Homework

When creating PCR reaction with small volumes we will make a Master Mix that can the combined with a PCR template. Complete the following calculations on a sample mix

$\backslash$table$[[$Master Mix,$\backslash$table$[[$Conc$.$ of stock$],[$solution$]],\backslash$table$[[$Final$],[$conc$.]],\backslash$table$[[$Volume $($uL$/$Rxn$)],[($total$/$tube$)]],\backslash$table$[[$Number of$],[$reactions$]],\backslash$table$[[$Total uL Needed$],[$for master mix$]]],[$PCR buffer,$10x,1x,,10,],[dNTP$ mix,$10mM,150uM,,,],[MgCl2,45mM,9mM,,,],[$Forward Primer,$20uM,2uM,,,],[$Reverse Primer,$20uM,2uM,,,],[,,0\mathrm{.}09,,,],[$Taq polymerase,$6\frac{U}{u}L,\frac{U}{u}L,,,],[$DNA$,,,1uL,,],[$Water$,,,,,],[$Total Reaction,,,$25uL,,],[$Volume$,,,,,]]$