Predict the phenotype for expression of the E$.$ coli trp operon enzymes $($in both Trp excess and Trp limited conditions$)$ for the following mutants:

a$)$ C to G substitution at nucleotide $22$ of the $trp$ operon leader RNA.

b$)$ Deletion of C at nucleotide $22$ of the trp operon leader RNA.

Explain your predictions taking into account the alternate stem$-$loop structures of the leader RNA. Assume the strain is $trpR-($i$.$e$.,$ no repressor$).$ A diagram of the $trp$ operon leader sequence and alternate stem$-$loop structures is shown on the accompanying sheet.

Remember that the bulk of a ribosome covers about $15$ nucleotides on either side of the triplet that the ribosome is sitting on$.$

trp operon leader RNA

$1$