Question: Pretend you are designing a protocol to extract chloroplasts from tomato leaves. It is imperative that you have as little contamination from nuclei and mitochondria

Pretend you are designing a protocol to extract chloroplasts from tomato leaves. It is imperative that you have as little contamination from nuclei and mitochondria as 2 possible. Using your knowledge of differential and rate-zonal centrifugation, write a protocol in point form which would achieve this. Refer to the figure and table below.

2.1 RNA 1.9 DNA 1.7 Ribosomes and polysomes 1.5 Soluble Nuclei Chloroplasts proteins Most viruses 1.3 Microsomes Mitocho

Table 1. Classes of centrifuges and their applications. Parameters Low speed High speed Ultracentrifuge Speed ranges (r.

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