Problem #1 (Manual Calculation") In a HPLC test, a liquid sample is analyzed for two specified ingredients, against a standard solution containing the respective ingredients in known concentrations. The individual ChemStation reports for the standard and sample injections are reproduced below (the chromatograms were recorded under identical chromatographic conditions): For the standard: RR HERRER Calibration Table (after recalibration) Calib. Data Modified t Friday, October 10, 2008 3:19:21 PM Level 1 calibrated Replace Response Factors, Replace Retention Times Signal 1. VD1 A, Wavelength-254 nm RetTime Lvl Amount Area Amt/Area Ref Grp Name (min) Sig Img/ml ---- |--1--1- ---- 0.527 1 1 1.02500 513.73914 1.995186-3 First compound 2.538 1 1 2.05500 2142.98877 9.589414-4 Second compound For the sample: External Standard 1 Sorted By Calib. Data Modified Multiplier Dilution Signal Friday, Oc 1.0000 1.0000 1 Signal 1: VWDL A, Wavelength-254 nm RetTime Type Area Amt/Area [min) (AUS) 0.526 BB 2.537 VB 516.22003 1.995180-3 2142.12549 9.589412-4 a). Identify the "given" quantities for external standard analysis in the ChemStation reports b). Calculate manually the concentrations of the two ingredients in the sample