Problem 1 Protein N-myristoylation involves the covalent attachment of the fatty acid, myristate (CH3(CH)12CO), to the...
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Problem 1 Protein N-myristoylation involves the covalent attachment of the fatty acid, myristate (CH3(CH₂)12CO₂¯), to the N-terminal glycine of a variety of cellular proteins. This type of reaction is one of many examples of a post-translational protein modification (or PTM). This particular reaction uses myristate-coenzyme A (myristate-SCOA) as a co-substrate and is catalyzed by an enzyme known as N-myristoyltransferase (NMT). ارسی یا H myristoyl-SCOA R +H3N NMT pH 7 요 12 N + HS H CoA-SH R + H+ (a) Propose two possible mechanisms for the N-myristoylation reaction. In one mechanism, assume there is an active site Cys and that the reaction occurs through a covalent intermediate. In the other, assume that a Glu residue is critical for enzyme catalysis. (b) Kinetics analysis of the myristoylation reaction catalyzed by NMT was performed by varying the concentration of myristoyl-CoA at fixed concentrations of a peptide substrate (panel A). The converse experiment in which peptide concentration is varied at fixed [myristoyl-CoA] was also conducted (panel B). From these data, estimate Kms for both myristoyl-CoA and the peptide substrate. (Note: assume that in both plots all lines converge to a single point on the x-axis.) A 1/v (1/pmol/min) -0.5 191 μM peptide O 96.4 μM peptide 47.7 μM peptide □23.8 μM peptide 0.5 1.0 1/[MyristoylCoA] (µM-1) 0.0 1.5 B 1/v (1/pmol/min) 0 ● 4.3 μM Myr-CoA 228 μM Myr-CoA 125 μM Myr-CoA 0.74 μM Myr-CoA -10 0 10 1/[GNAAAARR-NH₂) (μM-1) x 103 20 30 40 50 (c) Given the data in part (b), can you distinguish between the two mechanisms in part (a). Briefly explain your reasoning. Problem 1 Protein N-myristoylation involves the covalent attachment of the fatty acid, myristate (CH3(CH₂)12CO₂¯), to the N-terminal glycine of a variety of cellular proteins. This type of reaction is one of many examples of a post-translational protein modification (or PTM). This particular reaction uses myristate-coenzyme A (myristate-SCOA) as a co-substrate and is catalyzed by an enzyme known as N-myristoyltransferase (NMT). ارسی یا H myristoyl-SCOA R +H3N NMT pH 7 요 12 N + HS H CoA-SH R + H+ (a) Propose two possible mechanisms for the N-myristoylation reaction. In one mechanism, assume there is an active site Cys and that the reaction occurs through a covalent intermediate. In the other, assume that a Glu residue is critical for enzyme catalysis. (b) Kinetics analysis of the myristoylation reaction catalyzed by NMT was performed by varying the concentration of myristoyl-CoA at fixed concentrations of a peptide substrate (panel A). The converse experiment in which peptide concentration is varied at fixed [myristoyl-CoA] was also conducted (panel B). From these data, estimate Kms for both myristoyl-CoA and the peptide substrate. (Note: assume that in both plots all lines converge to a single point on the x-axis.) A 1/v (1/pmol/min) -0.5 191 μM peptide O 96.4 μM peptide 47.7 μM peptide □23.8 μM peptide 0.5 1.0 1/[MyristoylCoA] (µM-1) 0.0 1.5 B 1/v (1/pmol/min) 0 ● 4.3 μM Myr-CoA 228 μM Myr-CoA 125 μM Myr-CoA 0.74 μM Myr-CoA -10 0 10 1/[GNAAAARR-NH₂) (μM-1) x 103 20 30 40 50 (c) Given the data in part (b), can you distinguish between the two mechanisms in part (a). Briefly explain your reasoning.
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Related Book For
Fundamentals of biochemistry Life at the Molecular Level
ISBN: 978-0470547847
4th edition
Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt
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