Question: Problem 1. Show how you would make the following buffers from solid material 1A). 25mM Tris pH 8.00.02Sm121.1g/mu=3.0275gM=121.1g/mu 8.0=8.06+logBH+BlogBH+B=0.06H+=100=0.87[BH+]+H+=0.0250.87[BH+]+[H+]=0.025BH+=0.0133=0.0116 1B) 900ml of 20mM Hepes, 100mMNaCl,1mM

Problem 1. Show how you would make the following buffers from solid material 1A). 25mM Tris pH 8.00.02Sm121.1g/mu=3.0275gM=121.1g/mu 8.0=8.06+logBH+BlogBH+B=0.06H+=100=0.87[BH+]+H+=0.0250.87[BH+]+[H+]=0.025BH+=0.0133=0.0116 1B) 900ml of 20mM Hepes, 100mMNaCl,1mM DTT pH 8.5 1C) 8L of 20mM sodium acetate pH5.0 1D) 10mMNaClpH6.5

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