Question: PROBLEM 5 You are given a 4.0 ug/mL solution of an enzyme (MW = 40,000Da). In your experiment, you have determined the initial reaction velocities

PROBLEM 5 You are given a 4.0 ug/mL solution of an

PROBLEM 5 You are given a 4.0 ug/mL solution of an enzyme (MW = 40,000Da). In your experiment, you have determined the initial reaction velocities vo at several substrate concentrations, [S]. a. Determine the Km and Vm using lineweaver-Burke Plot b. Calculate the turnover number for the reaction S (MM) 0.25 0.40 0.70 1 2 3 5 8 vo (mM/min) 0.06 0.08 0.13 0.16 0.25 0.31 0.36 0.39

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