PROCEDURE\ Master Mix Worksheet & Difution Calculations:\ Using the Master Mix worksheet for general amplifications (

40\\\\mu l

and properly documenting ALL calculations in your notebook:\ a. Calculate the volumes needed per sample for each Master Mix component using the stock concentrations and final concentrations / total quantities indicated on the worksheet. Your final volume

(V_(2))

is

40H

for each component. Clearly show your work here:\ 10X PCR Buffer\

,2.0mM

dNTPs\

25mMMgCl