Question $2$

$1\mathrm{.}5$ pts

A mutation results in the change of Ser to Asp in the substrate binding pocket of chymotrypsin. Most likely, the mutant enzyme will

no longer catalyze the hydrolysis of a peptide bond because

Asp cannot facilitate the nucleophilic attack.

no longer catalyze the hydrolysis of a peptide bond because Asp is unable to be deprotonated by His$57.$

preferentially hydrolyze substrates containing phenylalanine.

preferentially hydrolyze substrates containing lysine.