QUESTION 2 (24 marks) In one study, a mixture containing neutral species (X: nonpolar, Y: relatively polar) and ionic species of different masses but similar charge (40p2., 50Q2, 50R2+, 5592+) was separated using capillary zone electrophoresis with a fused silica capillary. The instrument was set up with the detector placed at the cathode end. a) If the buffer of pH 2 is used: i) Explain whether or not all the analytes can be separated and detected. (5 marks) ii) With justification, suggest the order of elution analytes. (3 marks) b) If the pH of the buffer was above 3: i) Can all the analytes be separated and detected? Explain. (5 marks) ii) Predict the order of elution. Justify your answer. (5 marks) QUESTION 2 (24 marks) In one study, a mixture containing neutral species (X: nonpolar, Y: relatively polar) and ionic species of different masses but similar charge (40p2., 50Q2, 50R2+, 5592+) was separated using capillary zone electrophoresis with a fused silica capillary. The instrument was set up with the detector placed at the cathode end. a) If the buffer of pH 2 is used: i) Explain whether or not all the analytes can be separated and detected. (5 marks) ii) With justification, suggest the order of elution analytes. (3 marks) b) If the pH of the buffer was above 3: i) Can all the analytes be separated and detected? Explain. (5 marks) ii) Predict the order of elution. Justify your