Question 2 In a fed-batch culture operating with intermittent addition of glucose solution, values of the following parameters are given at time t = 2 h, when the system is at quasi-steady state. d V = 1000 ml F= = 200 ml/h dt -1 So = 100 g glucose/1 um = 0.3 h K, = 0.1 g glucose/1 YMS = 0.5 gdw cells/g glucose X/ X5 = 30 g a. Find V (the initial volume of the culture). b. Determine the concentration of growth-limiting substrate in the vessel at quasi-steady state. c. Determine the concentration and total amount of biomass in the vessel at t = 2 h (at quasi-steady state). d. If qp= 0.2 g product/g cells, Po = 0, determine the concentration of product in the vessel at t = 2 h