Question 3: (12 points) a). (3 points)What is the role of TISAB in the analysis of fluoride ions? (explain your answer) b). (3 points) What type of measurements can be obtained in lon selective electrode and what is a reference electrode? c). (3 points). Why the electrode slope is important? How the value of the electrode slope is obtained? d). (3 points) An ISE measurement was obtained for toothpaste sample to determine concentration F ions. A calibration line regression equation was generated, for three standard solutions of 1.00,5.00, and 10.0 ppm F in 100.0 mL volumetric flasks, and found to be linear in the following form: Y= 145.2-43.85log[F]. A 0.400 g sample of toothpaste was prepared in 50.0 mL and then 5.0 mL aliquot was diluted to 100.0 mL using TISAB and distilled water. Calculate the ppm of NaF in the toothpaste if the potentiometric value of the diluted solution is 148.5 mv. Please, show all your calculation to get full credits