Question $4$

You are mapping gene orders in bacteria using conjugation. Your recipient strain is negative for all traits listed in the columns of the table. You have three different Hfr donor strains that are positive for all those same traits. The table below summarizes the percent of recipient cells positive for each trait after $10,15,20,$ or $25$ minutes of conjugation with each of the three donors.

$4$a$.$ What is the apparent gene order?

Arg $--$

$4$b$.$ Which gene is closest to the origin of transfer in each of the Hfr strains?

Hfr$1=$

Hfr$2=$ type your answer...

Hfr$3=$ type your answer...