replicate1	replicate 2	replicate 3
blank	0.03320.0332	0.03300.0330	0.03350.0335
0.10 g/dL	0.08680.0868	0.08650.0865	0.08630.0863
0.20 g/dL	0.2060.206	0.2060.206	0.2060.206
0.50 g/dL	0.4650.465	0.4670.467	0.4660.466
1.00 g/dL	0.8980.898	0.8990.899	0.8970.897

A protein assay requires the reaction of the protein solution with a protein-binding dye for one minute, followed by measuring the absorbance of the protein-dye complex at 505 nm. The absorbance data for four standard protein solutions and the blank is provided in the table. The absorbance of each solution was measured three times. Construct a calibration curve using the data in the table. Find the slope (m), intercept (b), and their respective standard deviations using the method of least squares.

The calibration curve should be created from absorbance values that are corrected for the absorbance of the blank, and not the raw data in the table. Obtain the corrected data by subtracting the average absorbance of the three blank values from each absorbance value in the table, including the individual blank values. When creating the calibration curve, do not use the average absorbance at each protein concentration. Instead, each data point should be used separately, meaning there will be three data points at each protein concentration, for a total of 15 points, including each individual blank value.

m=

b=

Calculate the concentration and uncertainty of a protein solution that produced an average absorption of 0.5010.501 when measured 3 times.

concentration:

g/dL

g/dL