Question: Seeded BOD analyses were conducted on milk-processing wastewater. Three dilutions using 2.0 mL, 3.0 mL, and 4.0 mL portions of waste including 1.0 mL of

Seeded BOD analyses were conducted on milk-processing wastewater. Three dilutions using 2.0 mL, 3.0 mL, and 4.0 mL portions of waste including 1.0 mL of bacterial seed per 300 ml bottle were incubated and tested over a 7-day period. Blanks with 1.0 mL of seed diluted to 300 mL are also analyzed to permit correction for the contribution of the seed to sample. The initial DO concentration for all bottles based on the average of several tests was 8.2 mg/L. Sketch the BOD-time curves for each dilution series. Determine the 5-day BOD values and calculate the k rates using Thomas Slope method. The DO test results are as follows:

Time,

days

DO in Seed Test, mg/L

DO measurement in waste portions (mg/L)

2.0 mL

3.0 mL

4.0 mL

0

8.2

8.2

8.2

8.2

1

7.9

7.0

6.0

5.7

2

7.5

6.1

5.5

4.5

3

7.3

6.1

5.2

4.2

4

7.0

5.5

4.7

3.6

5

6.7

5.4

4.4

3.5

6

6.5

5.5

4.4

3.4

7

6.4

5.1

4.0

3.4

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