solve

You have a BSA solution with an unknown concentration. Do a 20-fold dilution of this solution in a final volume of 200 uL. Your DNA sample needs to get diluted by 10 fold before analysis, and the spectrophotometer cuvette must be filled with 50 pL of solution. Do the appropriate dilution. A stock bottle of chemical says that it is a "50x stock" (this is a common expression in labs; this solution is fifty-times more concentrated than the working solution). You need to make 1 L of the working solution (also called the "1x solution"). How would you make the 1x solution? How would you make 750 uL of a solution which is half as concentrated compared to the original? Yesterday, your experiment did not work since your sample was too concentrated. Your supervisor tells you "Try diluting the sample ten times and analyze it again. You'll need about 300 uL". Then, your supervisor says "Do it". You have a 10x stock solution which you need to dilute to a 1x solution. Go ahead and choose how much working solution you want to make. "Do it". You diluted a solution by adding 300 uL of the original solution to a total volume of 3000 UL. a. How much more dilute is the diluted solution as compared to the original? b. If the original solution was 50 mM CaCl2, what is the concentration of the diluted solution? You already know the dilution factor. Try to answer this question WITHOUT using an equation