Stoichiometric equations can be used to represent the growth of microorganisms provided a 'molecular formula' for...
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Stoichiometric equations can be used to represent the growth of microorganisms provided a 'molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O, and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30,2No.86. These bacterial cells are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is: C16H34 + 16.28 02 +1.42 NH3 1.65 C4.4H7.3012N0.86 + 8.74 CO2 + 13.11 H2O You have been put in charge of a small batch fermenter for growing the bacteria and aim to produce 8.2 kg of cells for inoculation of a pilot-scale reactor. a) What minimum amount of hexadecane substrate (in kg) must be contained in your culture medium? Assume 100% conversion of hexadecane to cells. b) What must be the minimum concentration of hexadecane (in kg/m) in the medium if the fermenter working volume is 5.8 cubic meters? c) What minimum volume (in m') of air at 30°C and 1.3 atm pressure must be pumped into the fermenter during growth to produce the required amount of cells? Stoichiometric equations can be used to represent the growth of microorganisms provided a 'molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O, and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30,2No.86. These bacterial cells are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is: C16H34 + 16.28 02 +1.42 NH3 1.65 C4.4H7.3012N0.86 + 8.74 CO2 + 13.11 H2O You have been put in charge of a small batch fermenter for growing the bacteria and aim to produce 8.2 kg of cells for inoculation of a pilot-scale reactor. a) What minimum amount of hexadecane substrate (in kg) must be contained in your culture medium? Assume 100% conversion of hexadecane to cells. b) What must be the minimum concentration of hexadecane (in kg/m) in the medium if the fermenter working volume is 5.8 cubic meters? c) What minimum volume (in m') of air at 30°C and 1.3 atm pressure must be pumped into the fermenter during growth to produce the required amount of cells?
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Answer a First of all the equation is balanced or not we have to observe The equation is C 16 H 34 1... View the full answer
Related Book For
Fundamentals of Electric Circuits
ISBN: 9780073301150
3rd edition
Authors: Matthew Sadiku, Charles Alexander
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