Question: What do you expect to see on your DNA gel if your colleague added the wrong restriction endonuclease to the strain Z sample, resulting in

What do you expect to see on your DNA gel if your colleague added the wrong restriction endonuclease to the strain Z sample, resulting in the plasmid being cut at only one site (and failing to release a fragment of interest)? 

Strain Z:  This E. coli strain contains a plasmid expressing the bacterial gene for CAT that confers resistance to chloramphenicol.

The strain Z sample would look like?

(a) (b) (c) (d) One band at the top, close to the

(a) (b) (c) (d) One band at the top, close to the well. One band far away from the well. at the bottom Two bands. No bands

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The correct option is a The band will appear at the top near to the well Because restriction digest ... View full answer

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